Authors

Sang Sun Yoon, Ray Coakley, Gee W. Lau, Sergei V. Lymar, Benjamin Gaston, Ahmet C. Karabulut, Robert F. Hennigan, Sung-Hei Hwang, Garry Buettner, Michael J. Schurr, Joel E. Mortensen, Jane L. Burns, David Speert, Richard C. Boucher, and Daniel J. Hassett

Details

Published in The Journal of Clinical Investigation, February 1, 2006

J Clin Invest. 2006 Feb 1; 116(2): 436–446.
PMID: 16440061, PMCID: PMC1350997
Published online 2006 Jan 26. doi: 10.1172/JCI24684
Read the full publication at the Journal of Clinical Investigation or at PMC NIH

Abstract

Mucoid, mucA mutant Pseudomonas aeruginosa cause chronic lung infections in cystic fibrosis (CF) patients and are refractory to phagocytosis and antibiotics. Here we show that mucoid bacteria perish during anaerobic exposure to 15 mM nitrite (NO₂^–) at pH 6.5, which mimics CF airway mucus. Killing required a pH lower than 7, implicating ...

Authors

Carmen L. Giltner, Erin J. Van Schaik, Gerald F. Audette, Dan Kao, Robert S. Hodges, Daniel J. Hassett, Randall T. Irvin

Details

First published in Molecular Microbiology, 12 December 2005, Volume 59, Issue 4 February 2006
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Abstract

Pseudomonas aeruginosa readily binds to stainless steel and other abiotic surfaces, causing major problems in both the medical and food industries. In this study, we show that P. aeruginosa binds to abiotic surfaces in a concentration-dependent, saturable manner during the initial stages of biofilm formation. P. aeruginosa type IV pili mediate binding to stainless steel as a pilus-deficient strain does not bind to steel, purified type IV pili bound in a concentration-dependent, saturable manner, and purified pili competitively inhibited whole cell binding. PAK pili can also bind polystyrene and polyvinylchloride in a concentration-dependant and saturable manner ...