Recent Publications
Peptide-Mediated PEGylation of Polysulfone Reduces Protein Adsorption and Leukocyte Activation
Authors
Noah G.G. Muruve, Y. Frank Cheng, Yuanchao Feng, Tao Liu, Daniel A. Muruve, Daniel J. Hassett, Randall T .Irvin
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Abstract
The exposure of blood to bio-incompatible materials used for dialysis triggers leukocyte activation and protein adsorption. We describe a single-step, post-manufacturing method for surface modification to create biomaterials used in medical devices and dialysis with altered surface characteristics. Peptides derived from the receptor-binding domain of the type IV pilin of Pseudomonas aeruginosa were synthesized using L and D-amino acids to generate L-K122-4, enantiomer D-K122-4, and D-retroinverso RI-K122-4 peptides. L-K122-4, D-K122-4, and RI-K122-4 peptides, but not control peptides, bound durably to the surfaces of materials used in medical devices and dialysis including silicone and polysulfone. D-K122-4 ...
A peptide-stainless steel reaction that yields a new bioorganic-metal state of matter
Authors
Elisabeth M Davis, Dong-Yang Li, Randall T Irvin
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Abstract
A synthetic peptide derived from the native protein sequence of a metal binding bacterial pilus was observed to spontaneously react with stainless steel via a previously unreported type of chemical interaction to generate an altered form of stainless steel which we term bioorganic stainless steel. Bioorganic stainless steel has a significantly increased electron work function (4.9 ± 0.05 eV compared to 4.79 ± 0.07 eV), decreased material adhesive force (19.4 ± 8.8 nN compared to 56.7 ± 10.5 nN), and is significantly harder than regular 304 stainless steel (~40% harder). A formal or semi-formal organo-metallic covalent bond is generated between a pilin receptor binding domain and stainless ...
NLRP3 inflammasome plays a key role in the regulation of intestinal homeostasis
Authors
Simon A. Hirota, PhD, Jeffrey Ng, MSc, Alan Lueng, Maitham Khajah, PhD, Ken Parhar, MD, Yan Li, MSc, Victor Lam, BSc, Mireille S. Potentier, Kelvin Ng, Misha Bawa, BSc, Donna-Marie McCafferty, PhD, Kevin P. Rioux, MD, PhD, Subrata Ghosh, MD, Ramnik J. Xavier, MD, Sean P. Colgan, PhD, Jurg Tschopp, PhD, Daniel Muruve, MD, Justin A. MacDonald, PhD, Paul L. Beck, MD, PhD
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Abstract
BACKGROUND: Attenuated innate immune responses to the intestinal microbiota have been linked to the pathogenesis of Crohn's disease (CD). Recent genetic studies have revealed that ...
